Biwako Sarutobi

Biwako had long, brown hair which she kept in a high ponytail, dark eyes, slightly visible creases at the corners of her eyes and her mouth. She wore a purple kimono-like dress, along with a white haori and sandals. As a child, Biwako's hair was shoulder-length with a pink flower hair band, she wore a green kimono, light-brown long sleeves shirt with a loquat on the back, red pants and sandals.

In the anime, it was shown that a young Biwako lived in the newly founded Konohagakure, where she attended the Academy. One day, she fell down in front of it and was comforted by Madara Uchiha, whose look frightened her deeply. Hashirama Senju, however, managed to calm her down by giving her a flower he received earlier. Biwako and her husband Hiruzen Sarutobi met with Kushina Uzumaki and Minato Namikaze shortly before Kushina was to give birth. Because Kushina was a jinchūriki, Hiruzen thought it best that Kushina gives birth at a secure location where Minato could maintain the seal, guarded by Anbu and with Biwako and Taji as her midwives. While they were on their way to the location, Kushina stopped to speak with Mikoto Uchiha who had just had her own baby. Biwako was pleasantly surprised that Mikoto had decided to name the boy after her husband's father but prompted Kushina to leave, warning her as they left that her delivery was top-secret. When Kushina went into labour, Biwako had to calm a hysterical Minato down as he maintained the seal, noting that women were a lot stronger than men. After delivering baby Naruto, Biwako was struck down by Obito Uchiha who had also killed all the Anbu stationed outside.